A large case-control study of gene expression and breast cancer death in the northern california kaiser permanente population
Expression of the 21 genes in the Recurrence Score assay and prediction of clinical benefit from tamoxifen in NSABP study B-14 and
chemotherapy in NSABP study B-20
Soonmyung Paik, MD 1, Steven Shak, MD 2, Gong Tang, PhD 1, Chungyeul Kim, MD 1, Heejae Joo, MD 1, Joffre Baker, PhD 2, Maureen
Cronin, PhD 2, Drew Watson, PhD 2, John Bryant, PhD 1, Joe Costantino, PhD 1 and Norman Wolmark, MD 1. 1 Operation and Biostatistics
Center, NSABP, Pittsburgh, PA, United States, 15212 and 2 Genomic Health Inc, Redwood City, CA, United States .
Two NSABP studies have been conducted to evaluate the 21 gene Recurrence Score (RS) assay as a predictor of distant
recurrence in patients with node negative, estrogen receptor positive breast cancer who were treated with tamoxifen alone. The study of 233
patients in the tamoxifen only arm of NSABP study B-20 was one of 3 studies conducted to select the 21 gene panel and define the
Recurrence Score algorithm. The large independent study of 668 tamoxifen-treated NSABP B-14 patients validated that the Recurrence
Score accurately predicts the likelihood of distant recurrence. To determine whether the Recurrence Score assay is predictive of tamoxifen or
chemotherapy benefit, we conducted two new studies of the Oncotype DXTM 21 gene assay.
Subjects and Methods:
1) The placebo arm patients in NSABP B-14 were studied to determine whether the genes in the Recurrence Score
assay predict pure prognosis, responsiveness to hormonal therapy, or both. 2) The patients in the tamoxifen plus chemotherapy arms of
NSABP B-20 were studied to determine whether expression of the genes in the Recurrence Score assay predict pure prognosis,
responsiveness to chemotherapy, or both. Patients were eligible if tumor blocks were available and contained at least 5% invasive cancer.
Based on tumor cellularity, RNA was extracted from three 10 micron sections without macrodissection or from macrodissected tumor from
six 10 micron sections (when tumor area was less than 50% of the section). Expression was quantified by RT-PCR for 16 cancer-related
genes (ER, PGR, BCL2, SCUBE2, Ki-67, MYBL2, Survivin, Cyclin B1, STK15, HER2, GRB7, Stromelysin 3, Cathepsin L2, GSTM1, BAG1, and CD68) and 5 reference genes (beta-actin, GAPDH, GUS, RPLPO, and TFRC).
: Study B-14. There were 645 evaluable patients (355 randomized to placebo and 290 randomized to tamoxifen). Results from
regression analysis indicate that the 21 gene Recurrence Score assay quantifies the likelihood of distant recurrence in node negative, ER +
breast cancer because it captures both prognosis and response to tamoxifen. Not all ER + patients benefited equally from tamoxifen. The
largest benefits of tamoxifen were observed with high quantitative ER (and generally low RS). Smaller benefits were observed with low
quantitative ER (and generally high RS). The tests for interaction between tamoxifen treatment and both RS and quantitative ER were
statistically significant (p<0.05). Study B-20. There were 651 evaluable patients (227 randomized to tamoxifen and 434 randomized to
tamoxifen plus either CMF or MF). Patients with tumors that had high Recurrence Scores (RS≥31) had a large absolute benefit of
chemotherapy (with an absolute increase in DRFS at 10 years of 27.6 ± 8.0%, mean±SE). Patients with tumors that had low Recurrence
Scores (RS<18) derived minimal, if any, benefit from chemotherapy (with an absolute increase in DRFS at 10 years of -1.1 ± 2.2%,
mean±SE). The test for interaction between chemotherapy treatment and RS was statistically significant (p<0.05).
The Oncotype DX Recurrence Score assay not only quantifies the likelihood of breast cancer recurrence in women with node
negative, estrogen receptor positive breast cancer, it also predicts the magnitude of chemotherapy benefit.
Kel y Goff Born 1980 Willemstad, Curaçao Netherlands Antilles Education Rhode Island School of Design Providence, RI Master of Fine Arts, Sculpture - With Honors 2009 New College of Florida Sarasota, FL Bachelor of Arts, Visual Art- Sub-concentration Biology/Chemistry 2002 Exhibitions The Wassaic Project Maxon Mills, Wassaic, NY 2012
October newsletter For Alpha Kindergarten (Fletton Setting) 2013 We would like to welcome all our new children and families throughout the nursery not forgetting the return of some families with their new additions! We have some exciting news that Lisa has had a baby girl called Paige and Jess has had baby boy congratulations to both families. For those of our family’s who do not know Lizz