Où achat cialis sans ordonnance et acheter viagra en France.

Aucft2003i 3_10

OGNEAN Claudia Felicia, DARIE Neli, OGNEAN Mihai “Lucian Blaga” University of Sibiu Abstract: This review summarizes information relate to new low-calorie sweetener:
erythritol, difructose anhydride, monellin, mabinlin, pentadin, and stevioside. In addition,
important developments in food applications are reported.

Dietary and health demands are continuing to expand the marked for
sweeteners as alternative to sucrose. Low-calorie sweeteners are important
for persons affected by diseases linked to the consumption of sugar, e.g.
diabetes, hyperlipemia, caries, overweight, obesity. Most sweet compounds,
including all popular sweeteners, are small molecular weight compounds of
widely different chemical nature, but there are also sweet macromolecules,
both synthetic and natural.
Erythritol is a 4-carbon sugar alcohol. Erythritol is a new potential sweet
food additive. Erythritol occurs widely in nature and has been found to occur
naturally in several foods including wine, sake, beer, water melon, pear,
grape and soy sauce at levels up to 0,13% (w/v) (Dubernet et al., 1974,
Shindou et al., 1989, Sponholz et al., 1986 quoted by Munro et al., 1998).
Evidences indicate that erythrol also exists endogenously in the tissues and
body fluids of humans and animals (Goosees and Röper, 1994; Noda et al.,
Owing to its properties, erythritol is intended for use as a low-calorie
sweetener. Its intended uses principally include confectionery, chewing gum,
and beverage and bakery products.
It has sweetness 60-80% of sucrose. It is produced from corn or wheat starch
by enzymatic hydrolysis yielding glucose which is fermented by safe and
suitable food-grade osmophilic yeast, either Moniliella pollinis or
Trichosporonoides megachliensis. Once erythritol is separated from the
ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 fermentation broth, it is purified to result in a crystalline product that is more
than 99% pure.
The safety database on erythritol provides no evidence that erythritol has any
carcinogenic, mutagenic or teratogenic potential. In addition, no effects on
reproductive performance or fertility have been reported in the studies. The
available studies demonstrate that erythritol is readily absorbed, is not
systemically metabolized, and is rapidly excreted unchanged in the urine.
Both animal toxicological studies and clinical studies have consistently
demonstrated the safety of erythritol, even when consumed on a daily basis
in high amounts (Munro et al., 1998).
Difructose anhydride is a new potential sweet food additive. The
disaccharide is derived from the vegetable storage compound inulin. It is a
non-reducing sugar which is very well soluble in water and has melting point
of 1620C. Due to an intramolecular dioxane ring (Fig. 1), the molecule is
very stable and is not hydrolysed in the stomach. It has approximately half
sweetness of sucrose, is not cariogenic, and is not metabolised by the human
body (Jahnz et al., 2003). As is known from oligofructoses, it influences the
composition of the intestinal flora positively as was shown in feeding
experiments with rats (Saito and Tomita, 2000). Likewise, in rats an
increased uptake of calcium could be observed in the intestine after
nourishing on difructose anhydride-enriched food. The authors conclude that
the risk of osteoporosis could be lowered by such a diet (Suzuki et al., 1998).
So far, difructose anhydride is not produced on a commercial scale as a food additive. Difructose anhydride can be produced either chemically or enzymatically from inulin. The prior method uses diluted sulfuric acid or pyrolysis conditions and yields of up to 40% can be found in inulin-derived caramels (Richards, 1996). The enzymatic route is more specific. The ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 enzyme inulase II (EC accomplished the difructose anhydride
formation by an intramolecular transfructosylation. This enzyme was first
described in Arthrobacter ureafaciens and has meanwhile been found in
various other bacteria (Kawamura et al., 1998, Yokota et al., 1991, Kim and
Lee, 2000). However, the cited enzymes are not long-term stable at elevated
temperature which is eligible for a process on industrial scale. To produce
difructose anhydride in technical and industrial scale, large amounts of
incapsulated enzyme are needed.
Monellin was first purified in 1972 by Morris and Cagan from the fruit of the
Dioscoreophylum cumminsii grown in West Africa. The fruit of 1 cm length
is found in grapelike clusters on the stem of the plant. The active ingredient
is isolated with sodium chloride solution and the extract is then ultrafiltered
and freeze-dried. It consist of two non-covalently associated polypeptide
chains, an A-chain of 44 residues and B-chain of 50 residues. These chains
have been completely sequenced by Frank and Zuber and Komura et al.
(Gibbs et al., 1996).
The molecular weight is 11 086 Da (5 251 from the A chain and 5 835 from
the B chain). The chains do not contain disulfide bonds or histidine. The
isoelectric point is between 9-9,4. The A chain does not contain any cysteine
while he B chain has one cysteine residue. Modification of the individual A
and B chains by replacement of peptides with cis-proline was found to
increase the thermostability of monellin (Kim and Lim, 1996).
Monellin is 100 000 times sweeter than sugar on a molar basis and several
thousand times on a weight basis. Monellin maintains its sweet taste between
pH 2,4-9,6, but it loses its taste at pH 10 due to unfolding of the tertiary
structure. Its activity is restored upon acidification. Denaturants such as urea
and sodium dodecyl sulfate lead to a complete loss in sweet taste. Subjecting
the protein to heat (pH 2) also caused a loss in sweetness but was recovered
by reneutralization. Since the sweet taste lasts for a long time, it has been
postulated that the binding of monellin to taste receptors is strong. In
addition, lactose and sucrose were found to inhibit binding (Konno et al.,
The native conformation is important for the sweet taste (Mizukoshi et al.,
1997). The commercial feasibility of monellin is very low despite its intense
sweetness. It is very costly to produce because the plant cannot be grown
outside of its natural environment. Its stability is limited. In addition, if
monellin is added to a carbonated drink such as cola, within a few hours, the
ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 sweetness will decrease rapidly (Suami et al., 1996). The taste properties are
also not ideal. Sweetness decline for up to an hour. In addition, proteolytic
enzymes decrease sweetness activity by creating peptides without sweetness.
However, modification of the protein may lead to a potentially commercial
product. Kim et al., (1998) were able to increase the sweetness of the protein
and stability towards pH and temperature changes by fusing the two chains
into one. This was accomplished by using several linkers from monellin
which were copied and then transplanted. The modified protein renaturated
after heating at low pH to 1000C.
In 1986, several sweet proteins were extracted from the seed of a plant found
in the south of China. The plant, Capparis masaikai, bears fruit as large as
apples and the natives have eaten the seeds. The proteins were called
mabinlins. Mabinlin was found to be 375 times sweeter than sucrose (Hu,
1986). These five homologous proteins were difficult to separate by
conventional techniques. This included extraction with sodium chloride and
ammonium sulphate, followed by ion-exchange and gel filtration
One of the proteins, designated mabinlin II, was found to be extremely heat
stable. It did not lose its sweetness after being subjected to 1000C for 48
hours. It is also the most abundant in seeds, 1,4 g being obtained from 100 g
of seeds. The molecular weight has been estimated at 14 kDa and the
isoelectric points as 11.3. This protein consist of two polypeptide chains,
chain A comprising 33 amino acids residues and chain B with 72 residues.
There is one disulfide bridge in chain A and 3 bridges in chain B. The chains
are non-covalently linked; the chain A contains mostly hydrophilic residues
while the Bcontains mostly hydrophobic. No homology was found between
mabinlin II and other sweet proteins (Liu et al., 1993).
In 1994, mabinlins I, III and IV were purified by Nirasawa et al. All Homologs were stable when heated at 800C for 1 hour with the exception of mabinlin I which lost its sweetness. An effort was then made to determine what the difference was between mabinlin I and the others by amino acid sequences and evaluation of the disulfide bridge position. The disulfide bridges were not responsible for the heat stability of the protein since they were the same in mabinlin I and mabinlin II. However, there were some differences in the amini acid sequences of the homologous proteins. It appears that the heat stability is due to the B-chain residue at position 47which is arginine in the heat stable proteins but is replaced by glutamine in ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 the unstable homolog. The arginine at position 47 may form a salt bridge
with the C-terminal of the A or B-chains (Nirasawa et al., 1994).
The most recently documented sweet protein was discovered in 1989 and
named pentadin. Microgram quantities were isolated from the extract of the
pulp of the plant Pentadiplandra brazzena Baillon. This plant found in
tropical Africa (especially Gabon) bears red globular berries approximately 2
inches in diameter. These berries contain one to five seeds surrounded by a
thick layer of pulp where the sweet protein resides. Ultrafiltration membrane
was used to eliminate the low molecular weight compounds. Then, gel
filtration was used to fractionate a series of proteins, one of which had an
intense sweet taste (Van der Wel, et al., 1989).
The substance was estimated to be 500 times as sweet as sucrose with a
similar taste profile to sweet proteins. The onset of sweetness due to sweet
proteins is relatively slow with a slight liquorice aftertaste. The sensation is
not only limited to the front of the tongue but over a large portion of the
tongue. The sweetness profile differs from sucrose and thus will probably be
used in combination with other sweeteners such as saccharin to mask the
bitter aftertaste of the latter (Gibbs et al., 1996).
Structurally, pentadin is believed to be a protomer whose smallest unit is
approximately 12 000 daltons molecular weight. Amino acid composition
revealed a high proline content, a residue associated with sweet taste. Other
dominant amino acids included aspartic acid, glutamic acid, tyrosine, lysine
and arginine. When are chemically denaturated, it retains its sweetness. It did
not lose its potency even after exposure to 1000C for 5 hours (Van der Wel,
et al., 1989). The presence of disulfide bonds was detected and may be
responsible for the protein’s stability. Because of the limited quantity
isolated, the protein was not further characterized.
Stevioside, a high intensity non-nutritive sweetener, is extracted from the
leaves of Stevia rebaudiana Bertoni, a sweet plant native to north-eastern
Paraguay. It is a white, crystalline, odourless powder which is approximately
300 times sweeter than sucrose (0,4% solutions). Structures of the sweet
components of Stevia occurring mainly in the leaves are given in Fig. 2.
Their content varies between 4 and 20 % of the dry weight of the leaves
depending on the cultivar and growing conditions. Stevioside 3 is the main
sweet component. Other compounds present but in lower concentrations are:
ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 steviolbioside 2, rebaudioside A 4, B 5, C 6, D 7, E 8, F 9 and dulcoside A 10 (Starrat et al., 2002). Stevia plant, its extracts, and stevioside have been used several years as a sweetener in South America, Asia, Japan, China, and in different countries of the EU. In Brazil, Korea and Japan Stevia leaves, stevioside and highly refined extracts are officially used as a low-calorie sweetener (Kim et al., 2002). In the USA, powdered Stevia leaves and refined extracts from the leaves have been used as a dietary supplement since 1995. In 2000, the European Commission refused to accept Stevia or stevioside as a novel food because of a lack of critical scientific reports on Stevia and the discrepancies between cited studies with respect to possible toxicological effects of stevioside and especially its aglycone steviol (Geuns, 2003). The advantages of stevioside as a dietary supplement for human subjects are mainfold: it is stable, it is non-calorific, it maintains good dental health by reducing the intake of sugar and opens the possibility for use by diabetic and phenylketonuria patients and obese persons. The stability of the low calorie sweetener stevioside during different processing and storage conditions, as well as the effects of its interactions with the water-soluble vitamins ascorbic acid, thiamin, ribiflavin, pyridoxine and nicotinic acid, the organic acids aceticacid, citric acid, tartric acid and phosphoric acid, the other common low calorie sweeteners saccharin, cyclamate, aspartame, acesulfame, neohesperidin dihydrochalcone, and caffeine in coffee and tea, were evaluated. Incubation of solid stevioside at high temperatures for 1 h showed good stability up to 1200C, whilst forced decomposition was noticed at temperatures exceeding 1400C. In aqueous solutions stevioside was remarkably stable in a pH range of 2-10 under thermal treatment up to 800C; however, under strong acidic conditions (pH 1), a significant decrese in the stevioside concentration was detected. Incubation up to 4 h of with individual water-soluble vitamins in aqueous solutions at 800C showed no significant changes with regard to stevioside and the B-vitamins, whereas a protective effect of stevioside on the degradation of ascorbic acid was observed, resulting in a significant delayed degradation rate. In the presence of other individual low calorie sweeteners, practically no interaction was found at room temperature after 4 month of incubation in aqueous media. Stability studies of stevioside in solutions of organic acids showed a tendency towards enhanced decomposition of the sweetener at lower pH values, depending on the acid medium. In stevioside-sweetened coffee and tea, very few significant chances in caffeine content or in stevioside content were found (Kroyer et al., 1999). ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 Fig. 2 – Structures of stevioside and related Table .1. Related compounds of stevioside Compound name
β-glucose-β-glucose (2→1) β-glucose-β-glucose (2→1) β-glucose (3→1) β-glucose-β-glucose (2→1) β-glucose (3→1) β-glucose-α-rhamnose (2→1) REFERENCES

1. B.F.Gibbs, and colab., Sweet and taste-modifying proteins: a review,1996,Nutrition
Research, vol. 16, nr. 9, pg. 1619-1630; 2. J.M.C.Geuns, Stevioside, 2003, Phytochemistry, nr.6; 3. J.Goossens, and H.Röper, Erythritol: A new bulk sweetener, 1994, International Food 4. Z.Hu, The sweet proteins from the seeds of Capparis masakai, 1986, Proceedings of the Munchen Shanghai Symposium: Peptide and Protein Chemistry; 5. U.Jahnz,et al., Process for producing the potential food ingredient DFA III from inulin: screening, genetic engineering, fermentation and immobilisation of inulase II, 2003, ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1 6. International Journal of Pharmaceutics, 7424, pg. 1-8; 7. M.Kawamura, S.Takahashi, T.Uchiyama, Purification and some properties of inulin fructotransferase (depolymeriying) from Arthrobacter ilicis – Agric. Biol. Chem., vol. 52, pp. 3209-3210; 8. S.H.Kim, et al., Redesigning a sweet protein: increased stability and renaturability, 9. G.E.Kim, S.Lee, Efficient production of DFA III (di-D-fructofuranose-dianhydride) from chicory root, 2000, Abstracts of the World Congress on Biotechnology 2000, vol. 272, Berlin; 10. I.H.Kim, K.J.Lim, Large-Scale Purification of Recombinant Monellin from Yeast, 1996, Journal of Fermentation and Bioengineering, vol. 82, nr. 2, pp. 180-182; 11. J.Kim, et al., Use of stevioside and cultivation of Stevia rebaudiana in Korea, 2002, Medicinal and Aromatic Plants – Industrial Profiles, vol. 19, pp. 196-202; 12. T.Konno, et al., Amyloid-like aggregates of a plant protein: a case of a sweet- asting protein, monellin, 1999, FEBS Letters, vol. 454, pp. 122-126; 13. G.T.Kroyer, The Lo7w Calorie Sweetener Stevioside: Stability and Interaction with Food Ingredients, 1999, Lebensm.-Wiss. u. Technol., vol. 32, pg. 509-512; 14. X.Liu, et al., Purification, complete amino acid sequence and structure characterization of heat-stable sweet protein, mabinlin II, 1993, Eur J Biochem, vol. 211, pp. 281-288; 15. T.Mizukoshi, et al., Structure and dynamic studies by NMR of the potent sweet protein monellin and a non-sweet analog, 1997, FEBS Letters, vol. 413, pp. 409-416; 16. I.C.Munro, et al., Erythritol: An Interpretive Summary of Biochemical, Metabolic, Toxicological and Clinical Data, 1998, Food and Chemical Toxicology, vol., 36, pp. 1139-1174; 17. S.Nirasawa, et al., Structures of heat-stable and unstable homologues of the sweet protein mabinlin. The difference in the heat stability is due to replacement of a single amino acid rezidue, 1994, Eur. J. Biochem, vol. 223, pp. 989-995; 18. K.Noda, K.Nakayama, and J.Modderman, Fate of erythritol after single oral administration to rats and dogs, 1996, Regulatory Toxicology and Pharmacology, vol., 24, pp. S206-S213; 19. G.N.Richards, Production of fructose dianhydride products from inulin, 1996, US- 20. K.Saito, F.Tomita, Difructose anhydrides: their mass-production and physiological functions, Biosci. Biotech. Biochem., vol. 64, pg. 1321-1327; 21. A.N.Starrat, et al., Rebaudioside F, a diterpene glycoside from Stevia rebaudiana, 2002, 22. T.Suami, et al., Molecular mechanism of sweet taste. Part 6: the sweet protein, Monellin, Food Chemistry, vol. 56, nr. 3, pp. 275-281; 23. T.Suzuki, et al., Effects of difructose anhydride III on calcium absorbtion in small and large intestin of rats, 1998, Biosci. Biotech. Biochem., vol. 62, pp. 837-841; 24. H.Van der Wel, et al., Isolation and characterization of pentadin, the sweet principle of Pentadiplandra brazzeana Baillon, 1989, Chemical Senses, vol. 14, pp. 75-79; 25. A.Yokota, et al., Production of inulin fructotransferase (depolymeriying) by Arthrobacter sp. H65-7 and preparation of DFA III from inulin by the enzyme, 1991, J. Ferm. Bioeng., vol., 72, pp. 258-261 ACTA UNIVERSITATIS CIBINIENSIS, 2003, no. 7, vol. 1

Source: http://saiapm.ulbsibiu.ro/rom/cercetare/ACTA_E/AUCFT2003I%203_10.pdf


dEL doMiNGo 2 AL SáBAdo 8 dE ENERo dE 2011 35 años, pero es entre 65 y 74 años donde se encuentran las diferencias más destacadas (88,6% en hombres y 81,5% en mujeres). También exis-ten diferencias en las frecuencias de medición del colesterol por clase so-cial, siendo las personas de las cla-ses sociales más altas quienes con más frecuencia se han hecho algu-na medición

Investigator – report for rulings panel

VERITEK LIMITED Notifying person CONTACT ENERGY LIMITED Participant allegedly in breach Breach Notice 2010-316 Record of settlement of alleged breach of rules 30, 31, 32, 33 of the Gas (Downstream Reconciliation) Rules 2008 11 March 2011 Record of settlement of alleged breach of rules 30, 31, 32, 33 of the Gas (Downstream Reconciliation) Rules 2008 Breach N

Copyright © 2010-2014 Pdf Medic Finder