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Results in Follicular Turnover in Crossbred
Estradiol benzoate administration at the start of a 7-d CIDR effectively initiated follicle turnover and tended to increase
pregnancy rates to timed-AI compared with no estradiol benzoate at CIDR insertion in crossbred Bos indicus cattle.
Crossbred lactating, postpartum cows (n=29) of Bos indicus breeding were used to determine the effectiveness of
estradiol benzoate to initiate follicle turnover when administered at the start of treatment with a 7-d intravaginalprogesterone -releasing device (CIDR). All cows received a CIDR with an injection of prostaglandin F2a (Lutalyse) atCIDR removal. At CIDR insertion, half of the cows received 2 mg of estradiol benzoate while the remainder did not.
Within the estradiol benzoate and no-estradiol benzoate treated groups, cows were timed-AI and injected withgonadotropin releasing hormone (GnRH - Cystorelin) at either 48 or 60 h after CIDR removal. Follicle turnover wasdetermined by ultrasonography. Estradiol benzoate treatment initiated follicle turnover in all (n=12) cows with a follicle > 10 mm. Cows treated with estradiol benzoate tended to have greater pregnancy rates (64.2 %) than cows not treated withestradiol benzoate (33.3%).
The utilization of artificial insemination (AI) in the beef cattle industry is becoming a more widely used production
practice. However, with timed-AI, or breeding by appointment, producers must utilize an effective estrus and/or ovulationcontrol system that provides consistent results. Adm inistration of progesterone-like compounds (progestagens) to cattlehave long been used to prevent cattle from exhibiting estrus. However, long term use (> 10 d) of progestagens results indevelopment of a large follicle that remains on the ovary for an extended period of time compared with what is typicallyobserved during the normal estrous cycle. The large follicle, known as persistent follicle, ovulates after removal of theprogestagen, but fertility of the subsequent breeding is decreased due to compromised development of the developingembryo. Furthermore, short term (< 7 d) progestagen treatments can result in ovulation of aged oocytes, which may also beof decreased fertility. Consequently, to prevent ovulation and fertilization of either a persistent follicle, or an aged oocyte,the follicle must be regressed either during or at the beginning a progestagen treatment so that a new follicle is recruited,selected, and ovulated at the estrus following withdrawal of the progestagen treatment. The objective of this study was todetermine the effectiveness of estradiol benzoate in combination with a 7-d progesterone treatment to initiate follicleturnover and to synchronize follicle development for a timed-AI in crossbred, lactating cows of Bos indicus breeding.
Crossbred, lactating, postpartum cows of Bos indicus breeding (n=29) were allotted to one of four treatments by
cycling status, days post-partum, and body condition score (1= emaciated; 5= moderate; 9= very fat). On the first day ofthe experiment, D 0, all cows received a 7-d CIDR (EAZI-BREEDTCIDRr, InterAg, Hamilton, NZ) and were injectedwith 5 mL of prostaglandin F2a (Lutalyse; Pharmacia & Upjohn, Kalamazoo, MI) at CIDR removal. Additionally, half ofthe cows were injected with 2 mL of estradiol benzoate (1 mg/mL; CIDROL, InterAg, Hamilton, NZ) at CIDR insertion
and half were not. Cows were timed-AI and simultaneously injected with 2 mL of GnRH (Cystorelin, Merial LimitedAthens, GA) at either 48 or 60 h after CIDR removal. Therefore, four AI treatments were represented in the study. Half of the cows received estradiol benzoate at insertion of the CIDR and half did not, and withineach of these groups, cows were timed-AI at either 48 or 60 h after CIDR removal. Estrus was detected using theHeatWatchr estrus detection system. Follicular development was determined using transrectal ultrasonography. On D 0 ofthe experiment ovaries were ultrasounded every other day until CIDR removal and then every day until ovulation. Thisallowed the researchers to determine if the estradiol benzoate caused the early regression of follicles, known as follicularturnover. Approximately 30 d after timed-AI, pregnancy was diagnosed with ultrasonography.
Estradiol benzoate administered on D 0 of the CIDR treatment initiated follicle turnover in all cows that had follicles
greater than 10 mm when the CIDR was inserted (Table 1). Of the cows that did not receive estradiol benzoate, only 7 of15 cows had follicle turnover during the duration of the CIDR. This is follicle turnover that occurs naturally in thefollicular cycle of the cow. Therefore, the injection of estradiol benzoate at the start of a 7-d CIDR treatment caneffectively initiate follicle turnover in cattle of Bos indicus breeding. Only 35.7 % of all animals in the experimentexhibited estrus during the period from CIDR withdrawal totimed-AI. Therefore, it appears that estrus expression iscompromised when cows are AI and simultaneously injected with GnRH at 48 or 60 h after CIDR removal.
Because there were no significant differences in pregnancy rates between cows that were AI at 48 vs. 60 h after CIDR
removal the data were combined. Cattle treated with estradiol benzoate tended to have greater (P < .09) AI pregnancy ratesthan cattle not treated with estradiol benzoate (64.3 vs. 33.3%; respectively). When the data were analyzed by cows thathad follicle turnover, regardless of whether the follicular turnover was natural or induced by estradiol benzoate, AIpregnancy rates was numerically greater for animals that had follicle turnover (10/19 = 52.6%) compared to follicles thatfailed to turnover (4/10 = 40.0%) during CIDR treatment.
In conclusion, estradiol benzoate administered at the time of CIDR insertion is an effective method of initiating
follicle turnover and may result in increased pregnancy rates in crossbred cattle of Bos indicus breeding.
1The CIDR has yet to be approved for use as an estrous synchronization agent by the Food and Drug Administration (FDA) in the
2The authors would like to thank InterAg, Hamilton New Zealand for donation of the CIDR and estradiol benzoate; Pharmacia
Upjohn for donation of the Lutalyse and Merial limited for donation of the Cystorelin. The authors would also like to thank Dr. Bryan Reilingand his Cow-Calf Management class for their assistance in conducting this project.
3Janis Fullenwider, graduate student; Ray Kempfer, former graduate student; Chris Barnett, former biological scientist; Joel Yelich,
assistant professor; Department of Animal Sciences, Gainesville.
Follicle turnover and follicle size at AI in cattle treated with estradiol benzoate at the time of CIDR insertion
(Includes follicles that were > 10 mm at CIDR insertion)
Size of follicles that
Size of follicles that
turned over at AI
failed to turnover at AI
Follicle turnover (%)
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